CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: UCH-L1]

CAT#: CF807197

Carrier-free (BSA/glycerol-free) CD45RO mouse monoclonal antibody, clone UCH-L1

Product Images

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-CD45RO mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA807197) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD45RO mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA807197) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45RO mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA807197) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD45RO mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA807197) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD45RO mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA807197) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD45RO mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (TA807197) (1:1000)

• Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-CD45RO antibody (TA807197), (Red), compared to a nonspecific negative control antibody ([TA180143]), (green) and PBS, (blue) (1:100).

• Flow cytometric Analysis of living Jurkat cells, using anti-CD45RO antibody (TA807197), (Red), compared to a nonspecific negative control antibody ([TA180143]), (green), and PBS, (blue) (1:100).

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Specifications

Product Data

• Clone Name: UCH-L1
• Applications: FC, IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: IL-2-dependent T-cell line (CA1).
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens protein tyrosine phosphatase receptor type C (PTPRC), transcript variant 2, mRNA.
• Database Link:
  NP_563578
  Entrez Gene 5788 Human
  UniProt ID P08575
• Background: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
• Synonyms: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway