pMirTarget 3'-UTR Assay Vector

Name: pMirTarget 3'-UTR Assay Vector
SKU: PS100062

CAT#: PS100062

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pMirTarget, the cloning vector for all 3'UTR clones for miRNA target validation, 10ug

Citations (45)

USD 570.00

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E. coli Selection	Kanamycin
Cell Selection	Neomycin
Features	pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3’UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity. High sensitivity of Luciferase assay due to IRES-driven luciferase cassette RFP as a reporter for transfection monitoring and normalization Neomycin selection marker for stable cell establishment Compatible with Precision shuttling system Can serve as a control for target validation experiments. 3-UTR Clones for MicroRNA target validation Download full vector sequence
Schematic of the multiple cloning sites

Citations (45)

The use of this Vectors has been cited in the following citations:
HMGA1-pseudogene7 transgenic mice develop B cell lymphomas ,De Martino, M;De Biase, D;Forzati, F;Credendino, SC;Palma, G;Barbieri, A;Arra, C;Paciello, O;Gaudio, E;Ponzoni, M;Vita, G;Chieffi, P;Bertoni, F;Fusco, A;Esposito, F;, Sci Rep 2020 ,PubMed ID 32341372 [pMirTarget]
MicroRNA-548-3p overexpression inhibits proliferation, migration, and invasion in osteoblast-like cells by targeting STAT1 and MAFB ,Ramírez-Salazar, EG;Almeraya, EV;López-Perez, TV;Patiño, N;Salmeron, J;Velázquez-Cruz, R;, J. Biochem. 2020 ,PubMed ID 32196088 [pMirTarget ]
Circulating microRNA-590-5p functions as a liquid biopsy marker in non-small cell lung cancer ,Khandelwal, A;Seam, RK;Gupta, M;Rana, MK;Prakash, H;Vasquez, KM;Jain, A;, Cancer Sci. 2020 ,PubMed ID 31520555 [pMirTarget]
The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b ,Chaudhuri, E;Dash, S;Balasubramaniam, M;Padron, A;Holland, J;Sowd, G;Villalta, F;Engelman, AN;Pandhare, J;Dash, C;, J. Biol. Chem. 2020 ,PubMed ID 32152226 [pMirTarget]
Activation of CB2R with AM1241 ameliorates neurodegeneration via the Xist/miR-133b-3p/Pitx3 axis ,He, X;Yang, L;Huang, R;Lin, L;Shen, Y;Cheng, L;Jin, L;Wang, S;Zhu, R;, J. Cell. Physiol. 2020 ,PubMed ID 31989652 [pMirTarget]
MiR-202-5p attenuates neurological deficits and neuronal injury in MCAO model rats and OGD-induced injury in Neuro-2a cells by targeting eIF4E-mediated induction of autophagy and inhibition of Akt/GSK-3β pathway ,Li, B;Huang, Z;Meng, J;Yu, W;Yang, H;, Mol. Cell. Probes 2019 ,PubMed ID 31877332 [pMirTarget]
Profiling microRNAs through development of the parasitic nematode Haemonchus identifies nematode-specific miRNAs that suppress larval development ,Marks, ND;Winter, AD;Gu, HY;Maitland, K;Gillan, V;Ambroz, M;Martinelli, A;Laing, R;MacLellan, R;Towne, J;Roberts, B;Hanks, E;Devaney, E;Britton, C;, Sci Rep 2019 ,PubMed ID 31772378 [pMirTarget]
miR-15a-5p, miR-15b-5p, and miR-16-5p inhibit tumor progression by directly targeting MYCN in neuroblastoma ,Chava, S;Reynolds, PC;Pathania, AS;Gorantla, S;Poluektova, LY;Coulter, DW;Gupta, SC;Pandey, MK;Challagundla, KB;, Mol Oncol 2019 ,PubMed ID 31637848 [pMirTarget]
Inhibition of miR30a-3p by sulforaphane enhances gap junction intercellular communication in pancreatic cancer ,Georgikou, C;Yin, L;Gladkich, J;Xiao, X;Sticht, C;Torre, C;Gretz, N;Gross, W;Schäfer, M;Karakhanova, S;Herr, I;, Cancer Letters 2019 ,PubMed ID 31678166 [pMirTarget]
miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling ,Wang, Q;Yu, X;Dou, L;Huang, X;Zhu, K;Guo, J;Yan, M;Wang, S;Man, Y;Tang, W;Shen, T;Li, J;, Oxidative Medicine and Cellular Longevity 2019 ,PubMed ID 31612078 [pMirTarget]
Macrophages exposed to HIV viral protein disrupt lung epithelial cell integrity and mitochondrial bioenergetics via exosomal microRNA shuttling ,Yuan, Z;Petree, JR;Lee, FE;Fan, X;Salaita, K;Guidot, DM;Sadikot, RT;, Cell Death Dis 2019 ,PubMed ID 31371699 [pMirTarget]
HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation ,Carelli, S;Giallongo, T;Rey, F;Latorre, E;Bordoni, M;Mazzucchelli, S;Gorio, MC;Pansarasa, O;Provenzani, A;Cereda, C;Di Giulio, AM;, RNA Biol 2019 ,PubMed ID 31345103 [pMirTarget]
Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer ,Fletcher, CE;Sulpice, E;Combe, S;Shibakawa, A;Leach, DA;Hamilton, MP;Chrysostomou, SL;Sharp, A;Welti, J;Yuan, W;Dart, DA;Knight, E;Ning, J;Francis, JC;Kounatidou, EE;Gaughan, L;Swain, A;Lupold, SE;de Bono, JS;McGuire, SE;Gidrol, X;Bevan, CL;, Oncogene 2019 ,PubMed ID 31043708 [pMirTarget]
24R,25-Dihydroxyvitamin D3 regulates breast cancer cells in vitro and in vivo ,Verma, A;Cohen, DJ;Schwartz, N;Muktipaty, C;Koblinski, J;Boyan, BD;Schwartz, Z;, Biochim Biophys Acta Gen Subj 2019 ,PubMed ID 31125679 [pMirTarget]
MicroRNA-365a-3p inhibits c-Rel-mediated NF-κB signaling and the progression of pancreatic cancer ,Yin, L;Xiao, X;Georgikou, C;Yin, Y;Liu, L;Karakhanova, S;Luo, Y;Gladkich, J;Fellenberg, J;Sticht, C;Gretz, N;Gross, W;Herr, I;, Cancer Lett. 2019 ,PubMed ID 30910589 [pMirTarget]
Regulation of collagen type XVII expression by miR203a-3p in oral squamous cell carcinoma cells ,Yasukochi, A;Kawakubo-Yasukochi, T;Morioka, M;Hazekawa, M;Nishinakagawa, T;Ono, K;Nakashima, M;Nakamura, S;, J. Biochem. 2019 ,PubMed ID 30918974 [pMirTarget]
MicroRNA-122 Mimic Improves Stroke Outcomes and Indirectly Inhibits NOS2 After Middle Cerebral Artery Occlusion in Rats ,Lv, B;Cheng, X;Sharp, FR;Ander, BP;Liu, DZ;, Front Neurosci 2018 ,PubMed ID 30405345 [pMirTarget]
Characterisation of infection associated microRNA and protein cargo in extracellular vesicles of Theileria annulata infected leukocytes ,Gillan, V;Simpson, DM;Kinnaird, J;Maitland, K;Shiels, B;Devaney, E;, Cell. Microbiol. 2018 ,PubMed ID 30370674 [pMirTarget]
MiR-335-5p restores cisplatin sensitivity in ovarian cancer cells through targeting BCL2L2 ,Liu, R;Guo, H;Lu, S;, Cancer Med 2018 ,PubMed ID 30019389 [pMirTarget]
MiR-126-3p inhibits ovarian cancer proliferation and invasion via targeting PLXNB2 ,Xiang, G;Cheng, Y;, Reprod Biol 2018 ,PubMed ID 30054097 [pMirTarget]
Inflammatory-Related P62 Triggers Malignant Transformation of Mesenchymal Stem Cells through the Cascade of CUDR-CTCF-IGFII-RAS Signaling ,Xin, X;Wang, C;Lin, Z;Xu, J;Lu, Y;Meng, Q;Li, X;Yang, Y;Zheng, Q;Gui, X;Li, T;Pu, H;Xiong, W;Li, J;Jia, S;Lu, D;, Mol Ther Nucleic Acids 2018 ,PubMed ID 29858072 [pMirTarget]
Long noncoding RNA HULC accelerates liver cancer by inhibiting PTEN via autophagy cooperation to miR15a ,Xin, X;Wu, M;Meng, Q;Wang, C;Lu, Y;Yang, Y;Li, X;Zheng, Q;Pu, H;Gui, X;Li, T;Li, J;Jia, S;Lu, D;, Mol. Cancer 2018 ,PubMed ID 29895332 [pMirTarget]
Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181 ,Ferguson, DC;Blanco, JG;, Pharm. Res. 2018 ,PubMed ID 29302759 [pMirTarget]
Conditional Disruption of miR17∼92 in Osteoclasts Led to Activation of Osteoclasts and Loss of Trabecular Bone In Part Through Suppression of the miR17-Mediated Downregulation of Protein-Tyrosine Phosphatase-oc in Mice† ,Lau, KW;Stiffel, V;Rundle, CH;Amoui, M;Tapia, J;White, TD;Sheng, MH;, JBMR Plus 2017 [pMirTarget]
Hypoxic lung cancer-secreted exosomal miR-23a increased angiogenesis and vascular permeability by targeting prolyl hydroxylase and tight junction protein ZO-1 ,Hsu, YL;Hung, JY;Chang, WA;Lin, YS;Pan, YC;Tsai, PH;Wu, CY;Kuo, PL;, Oncogene 2017 ,PubMed ID 28436951 [pMirTarget]
Angiogenesis-targeting microbubbles combined with ultrasound-mediated gene therapy in brain tumors ,Chang, EL;Ting, CY;Hsu, PH;Lin, YC;Liao, EC;Huang, CY;Chang, YC;Chan, HL;Chiang, CS;Liu, HL;Wei, KC;Fan, CH;Yeh, CK;, J Control Release 2017 ,PubMed ID 28408200 [pMirTarget]
Identification of novel fibrosis modifiers by in vivo siRNA silencing ,Vollmann, EH;Cao, L;Amatucci, A;Reynolds, T;Hamann, S;Dalkilic-Liddle, I;Cameron, TO;Hossbach, M;Kauffman, KJ;Mir, FF;Anderson, DG;Novobrantseva, T;Koteliansky, V;Kisseleva, T;Brenner, D;eld, JD;Burkly, LC;, olecular Therapy: Nucleic Acids 2017 [pMirTarget]
MicroRNA106a Regulates Matrix Metalloprotease 9 in a Sirtuin Dependent Mechanism ,Edatt, L;Maurya, AK;Raji, G;K, H;Kumar, SV;, J. Cell. Physiol 2017 ,PubMed ID 28233301 [pmirTARGET]
Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly Regulated by miR-126 in Primary Human Osteoblasts ,Strassburg, S;Nabar, N;Lampert, F;Goerke, SM;Pfeifer, D;Finkenzeller, G;Stark, GB;Simunovic, F;, J. Cell. Biochem 2016 ,PubMed ID 27958650 [pMirTarget]
Let-7 Status Is Crucial for PARP1 Expression in HER2-Overexpressing Breast Tumors ,Wielgos, ME;Rajbhandari, R;Cooper, TS;Wei, S;Nozell, S;Yang, ES;, Mol. Cancer Res 2016 ,PubMed ID 28031413 [pMirTarget]
Dual Anti-Inflammatory and Anti-Angiogenic Action of miR-15a in Diabetic Retinopathy ,Wang, Q;Navitskaya, S;Chakravarthy, H;Huang, C;Kady, N;Lydic, TA;Chen, YE;Yin, KJ;Powell, FL;Martin, PM;Grant, MB;Busik, JV;, EBioMedicine 2016 ,PubMed ID 27531575
miR-526b-3p functions as a tumor suppressor in colon cancer by regulating HIF-1a ,Zhang, R;Zhao, J;Xu, J;Wang, J;Jia, J;, Am J Transl Res 2016 ,PubMed ID 27398161
Rare FOXC1 variants in congenital glaucoma: identification of translation regulatory sequences ,Medina-Trillo, C;Aroca-Aguilar, JD;Méndez-Hernández, CD;Morales, L;García-Antón, M;García-Feijoo, J;Escribano, J;, Eur. J. Hum. Genet. 2016 ,PubMed ID 26220699 [pMirTarget]
Functional characterization of eight rare missense CYP1B1 variants involved in congenital glaucoma and their association with null genotypes ,Medina-Trillo, C;Ferre-Fernández, JJ;Aroca-Aguilar, JD;Bonet-Fernández, JM;Escribano, J;, Acta Ophthalmol Apr 2016 ,PubMed ID 27060699
Isolinderalactone enhances the inhibition of SOCS3 on STAT3 activity by decreasing miR-30c in breast cancer ,Yen, MC;Shih, YC;Hsu, YL;Lin, ES;, Oncology reports Mar 2016 ,PubMed ID 26707189 [PS100062]
Enhanced MAPK signaling drives ETS1-mediated induction of miR-29b leading to downregulation of TET1 and changes in epigenetic modifications in a subset of lung SCC ,Taylor, MA;Wappett, M;Delpuech, O;Brown, H;Chresta, CM;, Oncogene Jan 2016 ,PubMed ID 26776158 [PS100062]
Noninvasive, Targeted, and Non-Viral Ultrasound-Mediated GDNF-Plasmid Delivery for Treatment of Parkinson's Disease ,Fan, CH;Ting, CY;Lin, CY;Chan, HL;Chang, YC;Chen, YY;Liu, HL;Yeh, CK;, Sci Rep Jan 2016 ,PubMed ID 26786201 [PS100062]
ErbB2-intronic microRNA-4728: a novel tumor suppressor and antagonist of oncogenic MAPK signaling ,Schmitt, DC;Madeira da Silva, L;Zhang, W;Liu, Z;Arora, R;Lim, S;Schuler, AM;McClellan, S;Andrews, JF;Kahn, AG;Zhou, M;Ahn, EY;Tan, M;, Cell Death Dis May 2015 ,PubMed ID 25950472 [PS100062]
A novel member of the let-7 microRNA family is associated with developmental transitions in filarial nematode parasites ,Winter, AD;Gillan, V;Maitland, K;Emes, RD;Roberts, B;McCormack, G;Weir, W;Protasio, AV;Holroyd, N;Berriman, M;Britton, C;Devaney, E;, BMC Genomics Apr 2015 ,PubMed ID 25896062 [PS100062]
Hypo- and Hypermorphic FOXC1 Mutations in Dominant Glaucoma: Transactivation and Phenotypic Variability ,Medina-Trillo, C;Sánchez-Sánchez, F;Aroca-Aguilar, JD;Ferre-Fernández, JJ;Morales, L;Méndez-Hernández, CD;Blanco-Kelly, F;Ayuso, C;García-Feijoo, J;Escribano, J;, PLoS ONE Mar 2015 ,PubMed ID 25786029 [PS100062]
HuR Suppresses Fas Expression and Correlates with Patient Outcome in Liver Cancer ,Zhu, H;Berkova, Z;Mathur, R;Sehgal, L;Khashab, T;Tao, RH;Ao, X;Feng, L;Sabichi, AL;Blechacz, B;Rashid, A;Samaniego, F;, Mol. Cancer Res. Feb 2015 ,PubMed ID 25678597 [PS100062]
MicroRNA-940 suppresses prostate cancer migration and invasion by regulating MIEN1 ,Rajendiran, S;Parwani, AV;Hare, RJ;Dasgupta, S;Roby, RK;Vishwanatha, JK;, Mol. Cancer Nov 2014 ,PubMed ID 25406943 [TrueClone]
Identification of miRNAs that modulate glucocerebrosidase activity in Gaucher disease cells ,Siebert, M;Westbroek, W;Chen, YC;Moaven, N;Li, Y;Velayati, A;Saraiva-Pereira, ML;Martin, SE;Sidransky, E;, RNA Biol Oct 2014 ,PubMed ID 25584808 [GE100007]
Loss of MBNL Leads to Disruption of Developmentally Regulated Alternative Polyadenylation in RNA-Mediated Disease ,Batra, R;Charizanis, K;Manchanda, M;Mohan, A;Li, M;Finn, DJ;Goodwin, M;Zhang, C;Sobczak, K;Thornton, CA;Swanson, MS;, Mol. Cell Sep 2014 ,PubMed ID 25263597 [SC400919]
MicroRNA-200b Suppresses Arsenic-transformed Cell Migration by Targeting Protein Kinase Ca and Wnt5b-Protein Kinase Ca Positive Feedback Loop and Inhibiting Rac1 Activation ,Wang, Z;Humphries, B;Xiao, H;Jiang, Y;Yang, C;, J. Biol. Chem. May 2014 ,PubMed ID 24841200 [BCRT101]

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Vector Resources
Video Tutorial: Clone Selection Guide Product Video: Why Should You Choose TrueORF GOLD shRNA Selection Guide Protocol: Lentivirus packaging CRISPR Knockout kit manual CRISPR vector manual CRISPR Trangene Knockin at AAVS1 and ROSA26 loci manual CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) manual

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