UBA52 Mouse Monoclonal Antibody [Clone ID: OTI4F2]

CAT#: CF807200

Carrier-free (BSA/glycerol-free) UBA52 mouse monoclonal antibody, clone OTI4F2 (formerly 4F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA52 (RC214495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA52 (1:2000). Positive lysates LY425589 (100ug) and LC425589 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBA52 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807200) (1:150)

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Specifications

Product Data

• Clone Name: OTI4F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to residues near C-terminus of human UBA52 (NP_003324).
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.5 kDa
• Gene Name: Homo sapiens ubiquitin A-52 residue ribosomal protein fusion product 1 (UBA52), transcript variant 1, mRNA.
• Database Link:
  NP_001029102
  Entrez Gene 22186 MouseEntrez Gene 7311 Human
  UniProt ID P62987
• Background: Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]
• Synonyms: CEP52; HUBCEP52; L40; RPL40

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Ribosome