HIPK1 Mouse Monoclonal Antibody [Clone ID: OTI1G5]

CAT#: CF807257

Carrier-free (BSA/glycerol-free) HIPK1 mouse monoclonal antibody, clone OTI1G5 (formerly 1G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIPK1 (RC206176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIPK1 (1:2000). Positive lysates LY405750 (100ug) and LC405750 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1G5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 439-697 of human HIPK1(NP_852003) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87.1 kDa
• Gene Name: Homo sapiens homeodomain interacting protein kinase 1 (HIPK1), transcript variant 4, mRNA.
• Database Link:
  NP_852003
  Entrez Gene 15257 MouseEntrez Gene 365895 RatEntrez Gene 204851 Human
  UniProt ID Q86Z02
• Background: The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: Myak; Nbak2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors