SSX2IP Mouse Monoclonal Antibody [Clone ID: OTI1A6]

CAT#: CF807456

Carrier-free (BSA/glycerol-free) SSX2IP mouse monoclonal antibody, clone OTI1A6 (formerly 1A6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX2IP (RC207526, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX2IP. Positive lysates LY415525 (100ug) and LC415525 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-272 of human SSX2IP(NP_054740) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71.1 kDa
• Gene Name: Homo sapiens SSX family member 2 interacting protein (SSX2IP), transcript variant 5, mRNA.
• Database Link:
  NP_054740
  Entrez Gene 308023 RatEntrez Gene 117178 Human
  UniProt ID Q9Y2D8
• Background: This gene encodes a protein that binds the cancer-testis antigen Synovial Sarcoma X breakpoint 2 protein. The encoded protein may regulate the activity of Synovial Sarcoma X breakpoint 2 protein in malignant cells. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Oct 2009]
• Synonyms: ADIP; hMsd1

Reference Data

• Protein Pathways: Adherens junction