DR4 (TNFRSF10A) Mouse Monoclonal Antibody [Clone ID: OTI8A12]

CAT#: CF807496

Carrier-free (BSA/glycerol-free) TNFRSF10A mouse monoclonal antibody, clone OTI8A12 (formerly 8A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10A (RC202152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNFRSF10A.

• 

  Western blot analysis of extracts (10ug) from 1 cell line and 1 human tissue by using anti-TNFRSF10A monoclonal antibody at 1:200 dilution.

Specifications

Product Data

• Clone Name: OTI8A12
• Applications: WB
• Recommended Dilution: WB 1:200~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 263-468 of human TNFRSF10A(NP_003835) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens TNF receptor superfamily member 10a (TNFRSF10A), mRNA.
• Database Link:
  NP_003835
  Entrez Gene 8797 Human
  UniProt ID O00220
• Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]
• Synonyms: APO2; CD261; DR4; TRAILR-1; TRAILR1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity