LCOR Mouse Monoclonal Antibody [Clone ID: OTI2A9]

CAT#: CF807878

Carrier-free (BSA/glycerol-free) LCOR mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LCOR (RC224042, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LCOR (1:2000). Positive lysates LY410134 (100ug) and LC410134 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 204-433 of human LCOR(NP_115816) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.8 kDa
• Gene Name: Homo sapiens ligand dependent nuclear receptor corepressor (LCOR), transcript variant 1, mRNA.
• Database Link:
  NP_115816
  Entrez Gene 212391 MouseEntrez Gene 84458 Human
  UniProt ID Q96JN0
• Background: LCOR is a transcriptional corepressor widely expressed in fetal and adult tissues that is recruited to agonist-bound nuclear receptors through a single LxxLL motif, also referred to as a nuclear receptor (NR) box (Fernandes et al., 2003 [PubMed 12535528]). [supplied by OMIM, Mar 2008]
• Synonyms: MLR2

Reference Data

• Protein Families: Druggable Genome