ZNF69 Mouse Monoclonal Antibody [Clone ID: OTI3C3]

CAT#: CF808236

Carrier-free (BSA/glycerol-free) ZNF69 mouse monoclonal antibody, clone OTI3C3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF69 (RC208624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF69 (1:2000). Positive lysates LY411885 (100ug) and LC411885 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ZNF69 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808236) (1:150)

Show all 11 images

Specifications

Product Data

• Clone Name: OTI3C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZNF69(NP_068734) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.2 kDa
• Gene Name: Homo sapiens zinc finger protein 69 (ZNF69), transcript variant 2, mRNA.
• Database Link:
  NP_068734
  Entrez Gene 7620 Human
  UniProt ID Q9UC07
• Background: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]
• Synonyms: Cos5; hZNF3

Reference Data

• Protein Families: Transcription Factors