ATP6V1C2 Mouse Monoclonal Antibody [Clone ID: OTI8H4]

CAT#: CF808278

Carrier-free (BSA/glycerol-free) ATP6V1C2 mouse monoclonal antibody, clone OTI8H4 (formerly 8H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1C2 (RC204218, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1C2 (1:2000). Positive lysates LY408291 (100ug) and LC408291 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808278) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808278) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808278) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808278) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808278) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATP6V1C2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808278) (1:150)

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Specifications

Product Data

• Clone Name: OTI8H4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATP6V1C2(NP_653184) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.7 kDa
• Gene Name: Homo sapiens ATPase H+ transporting V1 subunit C2 (ATP6V1C2), transcript variant 2, mRNA.
• Database Link:
  NP_653184
  Entrez Gene 68775 MouseEntrez Gene 362802 RatEntrez Gene 245973 Human
  UniProt ID Q8NEY4
• Synonyms: ATP6C2; VMA5

Reference Data

• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection