RAB11FIP4 Mouse Monoclonal Antibody [Clone ID: OTI13B5]

CAT#: CF808377

Carrier-free (BSA/glycerol-free) RAB11FIP4 mouse monoclonal antibody, clone OTI13B5 (formerly 13B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB11FIP4 (RC210877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB11FIP4 (1:2000). Positive lysates LY409875 (100ug) and LC409875 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RAB11FIP4 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808377) (1:150)

Specifications

Product Data

• Clone Name: OTI13B5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 91-177 of human RAB11FIP4(NP_116321) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71.7 kDa
• Gene Name: Homo sapiens RAB11 family interacting protein 4 (RAB11FIP4), transcript variant 1, mRNA.
• Database Link:
  NP_116321
  Entrez Gene 268451 MouseEntrez Gene 84440 Human
  UniProt ID Q86YS3
• Background: Proteins of the large Rab GTPase family (see RAB1A; MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP4 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]). [supplied by OMIM, Apr 2008]
• Synonyms: RAB11-FIP4

Reference Data

• Protein Pathways: Endocytosis