NAPSIN A (NAPSA) Mouse Monoclonal Antibody [Clone ID: OTI1B5]

CAT#: CF808564

Carrier-free (BSA/glycerol-free) NAPSA mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPSA (RC204715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPSA (1:2000). Positive lysates LY401519 (100ug) and LC401519 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA808564) (1:400)

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 64-244 of human NAPSA(NP_004842) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.7 kDa
• Gene Name: Homo sapiens napsin A aspartic peptidase (NAPSA), mRNA.
• Database Link:
  NP_004842
  Entrez Gene 9476 Human
  UniProt ID O96009
• Background: The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq, Jul 2008]
• Synonyms: KAP; Kdap; NAP1; NAPA; SNAPA

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome