GAS8 Mouse Monoclonal Antibody [Clone ID: OTI10G2]

CAT#: CF808629

Carrier-free (BSA/glycerol-free) GAS8 mouse monoclonal antibody, clone OTI10G2 (formerly 10G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS8 (RC211150, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS8 (1:2000). Positive lysates LY419922 (100ug) and LC419922 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI10G2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GAS8 (NP_001472) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.2 kDa
• Gene Name: Homo sapiens growth arrest specific 8 (GAS8), transcript variant 1, mRNA.
• Database Link:
  NP_001472
  Entrez Gene 104346 MouseEntrez Gene 361438 RatEntrez Gene 2622 Human
  UniProt ID O95995
• Background: This gene includes 11 exons spanning 25 kb and maps to a region of chromosome 16 that is sometimes deleted in breast and prostrate cancer. The second intron contains an apparently intronless gene, C16orf3, that is transcribed in the opposite orientation. This gene is a putative tumor suppressor gene. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
• Synonyms: CILD33; DRC4; GAS11