PD L1 (CD274) Mouse Monoclonal Antibody [Clone ID: OTI9E12]

CAT#: CF808771

Carrier-free (BSA/glycerol-free) CD274 mouse monoclonal antibody, clone OTI9E12 (formerly 9E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

• 

  PD-L1 ELISA with 13G7 Capture (TA809809) and 9E12 Detection (TA808771) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

• 

  PD-L1 ELISA with 9E12 Capture (TA808771) and 4D4 Detection (TA507099) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

• 

  PD-L1 ELISA with 2C7 Capture (TA507087) and 9E12 Detection (TA808771) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

• 

  Immunocytochemistry staining of stable expression CD274 cells using anti-CD274 mouse monoclonal antibody (TA808771) (Left). The right is negative control. (1:5000) (1:2000)

• 

  Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody (TA808771), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100).

• Flow cytometric Analysis of stable expression CD274 (RC213071) in HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-CD274 antibody (TA808771) (1:100).

• PD-L1 Luminex ELISA with 9E12 Capture (TA808771) and 4D4 Detection (TA507099) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

• PD-L1 Luminex ELISA with 2C7 Capture (TA507087) and 9E12 Detection (TA808771) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

Show all 9 images

Specifications

Product Data

• Clone Name: OTI9E12
• Applications: ELISA, FC, IF, LMNX, WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-239 of human PD-L1/CD274(NP_054862) produced in HEK293 cells.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31 kDa
• Gene Name: Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.
• Database Link:
  NP_054862
  Entrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)