RPS6KC1 Mouse Monoclonal Antibody [Clone ID: OTI10F11]

CAT#: CF808825

Carrier-free (BSA/glycerol-free) RPS6KC1 mouse monoclonal antibody, clone OTI10F11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KC1 (RC211142, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KC1 (1:2000). Positive lysates LY402211 (100ug) and LC402211 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-RPS6KC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808825) (1:150)

Specifications

Product Data

• Clone Name: OTI10F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-305 of human RPS6KC1(NP_036556) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 118.5 kDa
• Gene Name: Homo sapiens ribosomal protein S6 kinase C1 (RPS6KC1), transcript variant 1, mRNA.
• Database Link:
  NP_036556
  Entrez Gene 26750 Human
  UniProt ID Q96S38
• Background: May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell. [UniProtKB/Swiss-Prot Function]
• Synonyms: humS6PKh1; RPK118; S6K-delta-1; S6PKh1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase