CEP55 Mouse Monoclonal Antibody [Clone ID: OTI6H3]

CAT#: CF808972

Carrier-free (BSA/glycerol-free) CEP55 mouse monoclonal antibody,clone OTI6H3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CEP55 (RC201052, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CEP55 (1:2000). Positive lysates LY413284 (100ug) and LC413284 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CEP55 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808972) (1:150)

Specifications

Product Data

• Clone Name: OTI6H3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-280 of human CEP55 (NP_060601) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens centrosomal protein 55 (CEP55), transcript variant 1, mRNA.
• Database Link:
  NP_060601
  Entrez Gene 55165 Human
  UniProt ID Q53EZ4
• Synonyms: C10orf3; CT111; URCC6

Reference Data

• Protein Families: Druggable Genome