GEF H1 (ARHGEF2) Mouse Monoclonal Antibody [Clone ID: OTI5F2]

CAT#: CF809320

Carrier-free (BSA/glycerol-free) ARHGEF2 mouse monoclonal antibody,clone OTI5F2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGEF2 (RC204546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGEF2 (1:2000). Positive lysates LY417793 (100ug) and LC417793 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of permeabilized Hela cells, using anti-ARHGEF2 antibody (TA809320), (Red), compared to negative control (PBS), (Blue) (1:100).

Specifications

Product Data

• Clone Name: OTI5F2
• Applications: FC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARHGEF2 (NP_004714) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 108.1 kDa
• Gene Name: Homo sapiens Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 3, mRNA.
• Database Link:
  NP_004714
  Entrez Gene 16800 MouseEntrez Gene 310635 RatEntrez Gene 9181 Human
  UniProt ID Q92974
• Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]
• Synonyms: GEF; GEF-H1; GEFH1; LFP40; P40

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection