XPB (ERCC3) Mouse Monoclonal Antibody [Clone ID: OTI6H8]

CAT#: CF809371

Carrier-free (BSA/glycerol-free) ERCC3 mouse monoclonal antibody,clone OTI6H8

Product Images

Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-ERCC3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ERCC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809371) (1:1000)

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Specifications

Product Data

• Clone Name: OTI6H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 191-436 of human ERCC3 (NP_000113) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89.1 kDa
• Gene Name: Homo sapiens ERCC excision repair 3, TFIIH core complex helicase subunit (ERCC3), transcript variant 1, mRNA.
• Database Link:
  NP_000113
  Entrez Gene 13872 MouseEntrez Gene 291703 RatEntrez Gene 2071 Human
  UniProt ID P19447
• Background: ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription. [provided by RefSeq, Jul 2008]
• Synonyms: BTF2; GTF2H; RAD25; TFIIH; TTD2; XPB

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair