GLS2 Mouse Monoclonal Antibody [Clone ID: OTI4C10]

CAT#: CF809494

Carrier-free (BSA/glycerol-free) GLS2 mouse monoclonal antibody,clone OTI4C10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLS2 (RC212650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLS2 (1:2000). Positive lysates LY415701 (100ug) and LC415701 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-GLS2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809494) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809494) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GLS2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809494) (1:2000)

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Specifications

Product Data

• Clone Name: OTI4C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human GLS2 (NP_037399) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.8 kDa
• Gene Name: Homo sapiens glutaminase 2 (GLS2), transcript variant 1, mRNA.
• Database Link:
  NP_037399
  Entrez Gene 192268 RatEntrez Gene 216456 MouseEntrez Gene 27165 Human
  UniProt ID Q9UI32
• Background: The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]
• Synonyms: GA; GLS; hLGA; LGA

Reference Data

• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism