NCR2 Mouse Monoclonal Antibody [Clone ID: OTI4B8]

CAT#: CF809536

Carrier-free (BSA/glycerol-free) NCR2 mouse monoclonal antibody,clone OTI4B8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NCR2 (Cat# RC214084, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCR2 (Cat# TA809536)(1:2000). Positive lysates LY417716 (100ug) and LC417716 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809536) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809536) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NCR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809536) (1:150)

Specifications

Product Data

• Clone Name: OTI4B8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 44-190 of human NCR2 (NP_004819) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.5 kDa
• Gene Name: natural cytotoxicity triggering receptor 2
• Database Link:
  NP_004819
  Entrez Gene 9436 Human
  UniProt ID O95944
• Synonyms: CD336; dJ149M18.1; LY95; NK-p44; NKP44

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Natural killer cell mediated cytotoxicity