Clathrin light chain (CLTA) Mouse Monoclonal Antibody [Clone ID: OTI2D12]

CAT#: CF809578

Carrier-free (BSA/glycerol-free) CLTA mouse monoclonal antibody,clone OTI2D12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CLTA (Cat# RC203505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLTA (Cat# TA809578)(1:2000). Positive lysates LY400696 (100ug) and LC400696 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines and mouse spleen tissue lysate by using anti-CLTA monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CLTA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809578) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLTA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809578) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CLTA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809578) (1:2000)

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Specifications

Product Data

• Clone Name: OTI2D12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CLTA(NP_001824) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: clathrin light chain A
• Database Link:
  NP_001824
  Entrez Gene 12757 MouseEntrez Gene 83800 RatEntrez Gene 1211 Human
  UniProt ID P09496
• Background: Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010]
• Synonyms: LCA

Reference Data

• Protein Pathways: Endocytosis, Huntington's disease, Lysosome