MAG1 (GPAT3) Mouse Monoclonal Antibody [Clone ID: OTI1D11]

CAT#: CF809765

Carrier-free (BSA/glycerol-free) AGPAT9 mouse monoclonal antibody,clone OTI1D11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGPAT9 (RC219236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGPAT9 (1:500). Positive lysates LY403194 (100ug) and LC403194 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines and rat brain tissue lysate by using anti-AGPAT9 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AGPAT9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809765) (1:500)

Specifications

Product Data

• Clone Name: OTI1D11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 41-131 of human AGPAT9 (NP_116106) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.5 kDa
• Gene Name: Homo sapiens glycerol-3-phosphate acyltransferase 3 (GPAT3), transcript variant 1, mRNA.
• Database Link:
  NP_116106
  Entrez Gene 84803 Human
  UniProt ID Q53EU6
• Synonyms: AGPAT8; AGPAT9; AGPAT 10; AGPAT10; HMFN0839; LPAAT-theta; MAG1

Reference Data

• Protein Families: Transmembrane