SIRT1 Mouse Monoclonal Antibody [Clone ID: OTI12H3]

CAT#: CF809859

Carrier-free (BSA/glycerol-free) SIRT1 mouse monoclonal antibody, clone OTI12H3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT1 (RC218134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT1 (1:2000). Positive lysates LY402173 (100ug) and LC402173 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI12H3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 552-676 of human SIRT1(NP_036370) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.5 kDa
• Gene Name: Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA.
• Database Link:
  NP_036370
  Entrez Gene 93759 MouseEntrez Gene 309757 RatEntrez Gene 23411 Human
  UniProt ID Q96EB6
• Background: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
• Synonyms: SIR2; SIR2alpha; SIR2L1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors