RBJ (DNAJC27) Mouse Monoclonal Antibody [Clone ID: OTI10A9]

CAT#: CF811078

Carrier-free (BSA/glycerol-free) DNAJC27 mouse monoclonal antibody,clone OTI10A9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJC27 (RC205500, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJ. Positive lysates LY413917 (100ug) and LC413917 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI10A9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNAJC27 (NP_057628) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.7 kDa
• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member C27 (DNAJC27), transcript variant 1, mRNA.
• Database Link:
  NP_057628
  Entrez Gene 217378 MouseEntrez Gene 298859 RatEntrez Gene 51277 Human
  UniProt ID Q9NZQ0
• Synonyms: RabJS; RBJ