ACADM Mouse Monoclonal Antibody [Clone ID: OTI10A5]

CAT#: CF811764

Carrier-free (BSA/glycerol-free) ACADM mouse monoclonal antibody,clone OTI10A5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACADM (RC202798, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACADM (1:2000). Positive lysates LY400001 (100ug) and LC400001 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ACADM monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811764) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811764) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811764) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811764) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811764) (1:500)

• Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-ACADM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811764) (1:500)

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Specifications

Product Data

• Clone Name: OTI10A5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACADM (NP_000007) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.59 kDa
• Gene Name: Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000007
  Entrez Gene 11364 MouseEntrez Gene 24158 RatEntrez Gene 34 Human
  UniProt ID P11310
• Background: This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: ACAD1; MCAD; MCADH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: beta-Alanine metabolism, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway, Propanoate metabolism, Valine, leucine and isoleucine degradation