RFC3 Mouse Monoclonal Antibody [Clone ID: OTI1C7]

CAT#: CF811943

Carrier-free (BSA/glycerol-free) RFC3 mouse monoclonal antibody,clone OTI1C7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC3 (RC201655, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC3. Positive lysates LY419018 (100ug) and LC419018 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 cell lines by using anti-RFC3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RFC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811943) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-RFC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811943) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RFC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811943) (1:500)

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Specifications

Product Data

• Clone Name: OTI1C7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RFC3 (NP_002906) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.4 kDa
• Gene Name: Homo sapiens replication factor C subunit 3 (RFC3), transcript variant 1, mRNA.
• Database Link:
  NP_002906
  Entrez Gene 69263 MouseEntrez Gene 288414 RatEntrez Gene 5983 Human
  UniProt ID P40938
• Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
• Synonyms: RFC38

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair