HSPA1L Mouse Monoclonal Antibody [Clone ID: OTI3B5]

CAT#: CF812122

Carrier-free (BSA/glycerol-free) HSPA1L mouse monoclonal antibody,clone OTI3B5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1L (RC205351, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1. Positive lysates LY417244 (100ug) and LC417244 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3B5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 400-641 of human HSPA1L (NP_005518) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.2 kDa
• Gene Name: Homo sapiens heat shock protein family A (Hsp70) member 1 like (HSPA1L), mRNA.
• Database Link:
  NP_005518
  Entrez Gene 15482 MouseEntrez Gene 24963 RatEntrez Gene 3305 Human
  UniProt ID P34931
• Background: This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008]
• Synonyms: HSP70-1L; HSP70-HOM; HSP70T; hum70t

Reference Data

• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome