Lymphocyte Activation Gene 3 (LAG3) Mouse Monoclonal Antibody [Clone ID: OTI7E3]

CAT#: CF813026

Carrier-free (BSA/glycerol-free) LAG3 mouse monoclonal antibody,clone OTI7E3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAG3 (RC220269, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAG3 (1:500).

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  Flow cytometric analysis of living 293T cells transfected with LAG3 overexpression plasmid (RC220269), Red)/empty vector (PS100001, Blue) using anti-LAG3 antibody (TA813026). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI7E3
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LAG3 (NP_002277) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.45 kDa
• Gene Name: Homo sapiens lymphocyte activating 3 (LAG3), mRNA.
• Database Link:
  NP_002277
  Entrez Gene 3902 Human
  UniProt ID P18627
• Background: Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProtKB/Swiss-Prot Function]
• Synonyms: CD223

Reference Data

• Protein Families: Transmembrane