MEPCE Mouse Monoclonal Antibody [Clone ID: OTI2E7]

CAT#: CF813128

Carrier-free (BSA/glycerol-free) MEPCE mouse monoclonal antibody,clone OTI2E7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEPCE (RC200948, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEPCE (1:500).

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  Western blot analysis of extracts (35ug) from 3 cell lines lysates by using anti-MEPCE monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI2E7
• Applications: WB
• Recommended Dilution: WB 1:250~500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-289 of human MEPCE (NP_062552) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.2 kDa
• Gene Name: Homo sapiens methylphosphate capping enzyme (MEPCE), transcript variant 1, mRNA.
• Database Link:
  NP_062552
  Entrez Gene 231803 MouseEntrez Gene 56257 Human
  UniProt ID Q7L2J0
• Synonyms: BCDIN3

Reference Data

• Protein Families: Druggable Genome