TNFSF9 Mouse Monoclonal Antibody [Clone ID: OTI3B6]

CAT#: CF813213

Carrier-free (BSA/glycerol-free) CD137L mouse monoclonal antibody,clone OTI3B6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD137L (RC211160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD137L (1:1000).

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  Flow cytometric analysis of living 293T cells transfected with CD137L overexpression plasmid (RC211160), Red)/empty vector (PS100001, Blue) using anti-CD137L antibody (TA813213). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living Raji cells, using anti-CD137L antibody (TA813213, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Specifications

Product Data

• Clone Name: OTI3B6
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD137L (NP_003802) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.4 kDa
• Gene Name: Homo sapiens TNF superfamily member 9 (TNFSF9), mRNA.
• Database Link:
  NP_003802
  Entrez Gene 8744 Human
  UniProt ID P41273
• Background: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction. [provided by RefSeq, Oct 2008]
• Synonyms: 4-1BB-L; CD137L; TNLG5A

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction