CD38 Mouse Monoclonal Antibody [Clone ID: OTI8D5]

CAT#: CF813842

Carrier-free (BSA/glycerol-free) CD38 mouse monoclonal antibody, clone OTI8D5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD38 (RC203179, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38.(1:1000)

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  Flow cytometric analysis of living 293T cells transfected with CD38 overexpression plasmid (RC203179), Red)/empty vector (PS100001, Blue) using anti-CD38 antibody (TA813842). Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）

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  Flow cytometric analysis of living Raji cells, using anti-CD38 antibody(TA813842, Red), compared to an isotype control (green).(1:100)

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  Flow cytometric analysis of living K562 cells, using anti-CD38 antibody(TA813842, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living RPMI-8226 cells, using anti-CD38 antibody(TA813842, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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  Flow cytometric analysis of living OPM2 cells, using anti-CD38 antibody(TA813842, Red), compared to an isotype control (green), and a PBS control (blue).(1:100)

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Specifications

Product Data

• Clone Name: OTI8D5
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD38 (NP_001766) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.3 kDa
• Gene Name: Homo sapiens CD38 molecule (CD38), transcript variant 1, mRNA.
• Database Link:
  NP_001766
  Entrez Gene 952 Human
  UniProt ID P28907
• Background: Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. [UniProtKB/Swiss-Prot Function]
• Synonyms: ADPRC 1; ADPRC1

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Calcium signaling pathway, Hematopoietic cell lineage, Metabolic pathways, Nicotinate and nicotinamide metabolism