bcl 6 (BCL6) Mouse Monoclonal Antibody [Clone ID: OTI8H3]

CAT#: CF813918

Carrier-free (BSA/glycerol-free) BCL-6 mouse monoclonal antibody, clone OTI8H3

Product Images

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, Cat# TA813918)

Specifications

Product Data

• Clone Name: OTI8H3
• Applications: IHC
• Recommended Dilution: IHC 1:400
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-350 of human BCL-6 (NP_001697) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.7 kDa
• Gene Name: Homo sapiens BCL6 transcription repressor (BCL6), transcript variant 1, mRNA.
• Database Link:
  NP_001697
  Entrez Gene 12053 MouseEntrez Gene 303836 RatEntrez Gene 604 Human
  UniProt ID P41182
• Background: The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
• Synonyms: BCL5; BCL6A; LAZ3; ZBTB27; ZNF51

Reference Data

• Protein Families: Druggable Genome, Transcription Factors