Ribonuclease H2, subunit A (RNASEH2A) Mouse Monoclonal Antibody [Clone ID: OTI7H10]

CAT#: CF815363

Carrier-free (BSA/glycerol-free) RNASEH2A mouse monoclonal antibody,clone OTI7H10

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human RNASEH2A plasmid (RC204032, lane 2), mouse RNASEH2A plasmid (MR204190, lane 3) using anti-ADAR antibody TA815363 (1:2000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts(50ug) from Jurkat cell lines lysate by using anti-RNASEH2A monoclonal antibody. (TA815363, 1:500)

Specifications

Product Data

• Clone Name: OTI7H10
• Applications: WB
• Recommended Dilution: WB1:500-1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RNASEH2A (NP_006388) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.4 kDa
• Gene Name: Homo sapiens ribonuclease H2 subunit A (RNASEH2A), mRNA.
• Database Link:
  NP_006388
  Entrez Gene 69724 MouseEntrez Gene 10535 Human
  UniProt ID O75792
• Background: The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid.[provided by RefSeq, Aug 2009]
• Synonyms: AGS4; JUNB; RNASEHI; RNHIA; RNHL; THSD8

Reference Data

• Protein Pathways: DNA replication