Ly6g Rat Monoclonal Antibody [Clone ID: RB6-8C5]

CAT#: CL046PT

Ly6g rat monoclonal antibody, clone RB6-8C5, Purified

Specifications

Product Data

• Clone Name: RB6-8C5
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Western Blotting.
  Immunoprecipitation.
  Flow Cytometry: Use 10 µl of 1/100-1/200 to label 10⁶ cells in 100 µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Normal murine bone marrow cells.
• Specificity: This antibody recognizes the Mouse Gr-1 antigen.
  Clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated.
  This Clone RB6-8C5 has been reported to deplete mouse mature neutrophils in vivo (3). We recommend the use of CL046LE for functional studies.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction from tissue culture supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography on Protein G
• Gene Name: lymphocyte antigen 6 complex, locus G
• Database Link:
  Entrez Gene 546644 Mouse
• Background: The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported.
• Synonyms: Gr-1 Granulocyte marker