Macrophages Rabbit Polyclonal Antibody

CAT#: CL194P

Macrophages rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: FN, IHC
• Recommended Dilution: Cytotoxic Assays.
  Immunohistochemistry on Frozen and Paraffin Sections.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: CL194P is directed against Mouse Macrophages.
  This antiserum is adsorbed repeatedly with Mouse Red Blood Cells, thymocytes and spleen cells to remove most antibodies to antigens common among different cell types. This adsorption significantly improves cytotoxic specificity.
• Formulation: State: Aff - Purified
  State: Liquid serum (0.2 μm filtered) without preservatives
• Synonyms: Macrophage marker
• Note: Protocol: Test Type:
  
  For Cytotoxic Antibodies:
  Modified Colorimetric Microtiter Assay (Ref.1)
  Results:
  Antisera of this lot diluted 1/20 exhibits greater than 75% cytotoxicity on Mouse Macrophages.
  Antisera of this lot diluted 1/20 exhibits less than 10% cytotoxicity on Mouse thymocytes or splenocytes.
  
  For Agglutinating Antibodies:
  Antisera dilutions in RPMI-1640 incubated with target cells at 4-8°C for 1hr. Agglutination determined by microscopic observations.
  Results:
  Antisera of this lot strongly agglutinates Mouse Macrophages at a dilution of 1/50.