Influenza M1 / HLA-A2 Complex (Extracell. Dom.) Mouse Monoclonal Antibody [Clone ID: 405H1.01]
CAT#: DDX0270P-100
Influenza M1 / HLA-A2 Complex (Extracell. Dom.) mouse monoclonal antibody, clone 405H1.01
Specifications
Product Data | |
Clone Name | 405H1.01 |
Applications | ELISA, FC |
Recommended Dilution | DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA. DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry. DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry.. DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry. DDX0270B-50 / DDX0270B-100 Biotin: ELISA. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | T2 cell line charged in M1 peptide |
Specificity | Human extracellular/M1 HLA neuropilin-1(epitope in extracellular domain). |
Formulation | Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8. Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol. State: Purified |
Concentration | 0.5 mg/ml |
Purification | QMA Hyper D ion exchange chromatography |
Background | In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule. |
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in shipping.