HIV-1 (gp120/160) Mouse Monoclonal Antibody [Clone ID: 115F10.05]

CAT#: DDX1302B-100

HIV-1 (gp120/160) mouse monoclonal antibody, clone 115F10.05

Product Images

IF staining of CHO-SEC with 115F10.05

• 

  gp120 detection in CHO-SEC supernatantby sandwich ELISA

Specifications

Product Data

• Clone Name: 115F10.05
• Applications: ELISA, FC, IF
• Recommended Dilution: DDX1302P-50 / DDX1302P-100 Purified: ImmunoFluorescence, Flow cytometry, detection ELISA gp120.
  DDX1302B-50 / DDX1302B-100 Biotin (on request): ImmunoFluorescence, Flow cytometry, detection ELISA gp120.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-25 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: gp160 (HIVIIIB from Biomérieux), gp120 (BAL from NIH), and CHO-NL4.3 cells
• Specificity: HIV gp120 and gp160.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  Label: Biotin
• Concentration: 0.5 mg/ml
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Biotin
• Background: HIV env gene encodes for a primary env product (gp160) which is cleaved into gp120 and gp41. gp120 is anchored to the viral membrane, via non-covalent bonds with the transmembrane glycoprotein, gp41. Mice were immunized with gp160 and gp120 recombinant proteins and the CHO-NL4.3 line expressing gp120 and the extracellular domain of gp41.
• Synonyms: HIV-I, HIV1, Human immunodeficiency virus type 1