Aflatoxin (AFM1+AFB1) Rat Monoclonal Antibody [Clone ID: 1C6]

CAT#: DM1000

Aflatoxin (AFM1+AFB1) rat monoclonal antibody, clone 1C6, Purified


USD 620.00

2 Weeks*

Size
    • 500 ug

Product Images

Specifications

Product Data
Clone Name 1C6
Applications ELISA
Recommended Dilution ELISA (Indirect): Reactive to Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1). 
Fluorimetric Immunoassay: Reactive to Aflatoxin M1 (AFM1) and Aflatoxin M2 (AFM2) (See Reference 1).
Host Rat
Isotype IgG2b
Clonality Monoclonal
Immunogen Aflatoxin M1-BSA conjugate
Specificity The selected monoclonal antibodies are reactive to Aflatoxin M1 (AFM1) and Aflatoxin B1 (AFB1), but not reactive to BSA and other irrelevant antigens by ELISA.
Similarly, a competitive binding assay using AFM1 as binding competitor to compete with AFM1-BSA-125I showed that AFM1 can effectively inhibit the binding of this monoclonal to AFM1-BSA-125I and the inhibition degree corresponded to the amounts of AFM1 used.
Formulation 0.01M PBS, pH 7.2 without preservatives
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore in double distillated water to adjust the final concentration to 1.0 mg/ml
Concentration lot specific
Purification Affinity Chromatography on protein G
Conjugation Unconjugated
Storage

Prior to reconstitution store at 2-8°C for one month or at -20°C for longer.
Following reconstitution store undiluted at 2-8°C for two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Background The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA and protein. Cytochrome P450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1 molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.