IL8 (CXCL8) Mouse Monoclonal Antibody [Clone ID: I8-60]
Specifications
Product Data | |
Clone Name | I8-60 |
Applications | ELISA, NEUT, WB |
Recommended Dilution | ELISA: Can be used as a Tracer antibody in Sandwich ELISA for Detection of Human IL-8 (matched pair with clone I8-S2, Cat.-No DM1022 as Capture antibody) Suggested Capture Coating Dose: 0.3 µg/well, substrate TMB. Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5 µg/ml of MAb and 10 ng/ml of Human IL-8 in assay. This MAb inhibited the chemotactic effect of Human IL-8, had no inhibitory effect to MIP 1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS. Western Blot: Concentration of 0.02-0.1 µg/ml MAb will allow visualization of 100 ng/lane of Human IL-8. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Purified recombinant human IL-8. |
Specificity | This monoclonal antibody recognizes both recombinant and native Human Interleukin-8. This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). |
Formulation | 0.01M PBS, pH 7.0 State: Azide Free State: Lyophilized purified Ig fraction |
Reconstitution Method | Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml. |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | C-X-C motif chemokine ligand 8 |
Database Link | |
Background | Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation. |
Synonyms | CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1 |
Reference Data |
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