VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: FLTEWF]

CAT#: DM3506B

VEGF Receptor 1 (FLT1) mouse monoclonal antibody, clone FLTEWF, Biotin

Specifications

Product Data

• Clone Name: FLTEWF
• Applications: ELISA, IF, IP, WB
• Recommended Dilution: ELISA (1-10 µg/ml).
  Western blotting (1-10 µg/ml).
  Immunofluorescence.
  Immunoprecipitation (2-10 µg/ml lysate or reaction volume).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539)
• Specificity: The unconjugated monoclonal antibody will recognize recombinant and naturally occurring form of VEGFR-1/Flt-1.
• Formulation: PBS
  Label: Biotin
  State: Lyophilized purified IgG fraction
  Stabilizer: 50X BSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 2321 Human
• Background: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
• Synonyms: VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1