VEGF Receptor 2 (KDR) Rabbit Polyclonal Antibody

CAT#: DP3509B

VEGF Receptor 2 (KDR) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, FN, IP, WB
• Recommended Dilution: ELISA: 5-15 μg/ml.
  Western blot.
  Immunoprecipitation.
  Neutralizing experiments.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant human soluble extracellular domain of KDR (110 kDa)
• Specificity: The antibody will detect native and recombinant VEGFR-2/KDR.
• Formulation: PBS
  Label: Biotin
  State: Lyophilized Ig fraction
  Stabilizer: 50x BSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Antigen Affinity chromatography with immobilised recombinant soluble VEGFR-2/KDR
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 3791 Human
• Background: VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
• Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2