Csf2 Rabbit Polyclonal Antibody

CAT#: PP003B2

Csf2 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA:
  (Direct): To detect mGM-CSF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF.
  (Sandwich): To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine GM-CSF (PP003P1 or PP003P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF.
  Western Blot:
  To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant mGM-CSF (murine GM-CSF)
• Specificity: Detects Murine GM-CSF.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration
  of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Biotin
• Database Link:
  Entrez Gene 12981 Mouse
• Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
• Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin
• Note: Centrifuge vial prior to opening!