Il1a Rabbit Polyclonal Antibody

CAT#: PP005B2

Il1a rabbit polyclonal antibody, Biotin


USD 355.00

2 Weeks*

Size
    • 50 ug

Product Images

Specifications

Product Data
Applications ELISA, WB
Recommended Dilution ELISA:
(Direct): To detect mIL-1α by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2- 0.4 ng/well of recombinant mIL-1α.
(Sandwich): To detect mIL-1α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-1α (PP005P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1α.
Western blot:
To detect mIL-1α by Western blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant mIL-1 alpha
Specificity This antibody reacts with Mouse Interleukin-1 alpha (IL-1 alpha).
Formulation PBS, pH 7.2 without preservatives
Label: Biotin
State: Lyophilized purified Ig fraction
Label: conjuated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration
of 0.1-1.0 mg/ml.
Purification Affinity chromatography
Conjugation Biotin
Background Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Synonyms IL-1 alpha, IL1F1, IL1A, Hematopoietin-1
Note Centrifuge vial prior to opening!
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.