Il1a Rabbit Polyclonal Antibody

CAT#: PP005P1

Il1a rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization:
  To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1 alpha (0.05 ng/ml), a concentration of 0.03-0.05 µg/ml of this antibody is required.
  ELISA:
  (Indirect): To detect mIL-1 alpha by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1 alpha.
  (Sandwich): To detect mIL-1 alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1 alpha (PP005B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1 alpha.
  Western blot:
  To detect mIL-1 alpha by Western blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant mIL-1-alpha (murine IL-1 alpha).
• Specificity: This antibody recats with Murine-1 alpha.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography
• Database Link:
  Entrez Gene 16175 Mouse
• Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
• Synonyms: IL-1 alpha, IL1F1, IL1A, Hematopoietin-1
• Note: Centrifuge vial prior to opening!