LOC105242482 Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | ELISA: To detect mMCP-5 by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivities | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant mMCP-5. |
Specificity | Recognises Macrophage Chemotactic Protein-5 (MCP-5) |
Formulation | PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated |
Reconstitution Method | Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity chromatography. |
Conjugation | Biotin |
Database Link | |
Background | MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations. |
Synonyms | MCP5, CCL-12, C-C motif chemokine 12. Macrophage Chemotactic Protein-5 |
Note | Centrifuge vial prior to opening! |
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