Tumor necrosis factor (TNF-alpha) Rabbit Polyclonal Antibody

CAT#: PP019B2

Tumor necrosis factor (TNF-alpha) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Mouse TNF-alpha by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse TNF-alpha.
  Sandwich ELISA: To detect Mouse TNF-alpha by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with a purified antibody (Cat.-No PP019P) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse TNF-alpha.
  Western Blot: To detect Mouse TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse TNF-alpha (Cat.-No PA031).
• Specificity: Reacts with Mouse TNF-alpha. Other species not tested.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Background: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin