Cntf Rabbit Polyclonal Antibody

CAT#: PP030B1

Cntf rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA
  Direct: To detect Rat CNTF by indirect ELISA (using 100 ml/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF.
  Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 ml/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. The Biotinylated antibody in conjunction with Anti-Rat CNTF (PP030P1 or PP030P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF.
  Western Blot
  To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified recombinant CNTF.
• Specificity: This antibody recognises Rat CNTF.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Gene Name: Rattus norvegicus ciliary neurotrophic factor (Cntf)
• Database Link:
  Entrez Gene 25707 Rat
• Background: CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.
• Synonyms: Ciliary Neurotrophic Factor