CXCL5 Rabbit Polyclonal Antibody

CAT#: PP1003B1

CXCL5 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA:
  (Direct): To detect hENA-78 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hENA-78.
  (Sandwich): To detect hENA-78 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human ENA-78 (PP1003P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hENA-78.
  Western Blot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hENA-78 (human ENA-78).
• Specificity: This antibody reacts with human Epithelial Neutrophil Activity Protein-78 (hENA-78)
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatcht.
• Database Link:
  Entrez Gene 6374 Human
• Background: CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
• Synonyms: C-X-C motif chemokine 5, Small-inducible cytokine B5, ENA-78, SCYB5, Cytokine LIX
• Note: Centrifuge vial prior to opening!