G CSF (CSF3) Rabbit Polyclonal Antibody

CAT#: PP1011P1

G CSF (CSF3) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: This G-CSF antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.

• 

Specifications

Product Data

• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human G-CSF (0.5 ng/ml), a concentration of 0.04-0.06 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Human G-CSF by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (Cat.-No PP1011B1 or PP1011B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human G-CSF.
  Western Blot: To detect Human G-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunohistochemistry: 0.25 µg/ml with an Overnight Incubation at 4°C.
  This antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human angiosarcoma. An HRP-labeled polymer detection system was used with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified E.Coli derived recombinant Human G-CSF (Cat.-No PA060).
• Specificity: Recognizes Human Granulocyte Colony Stimulating Factor (G-CSF).
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized (Sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized hG-CSF matrix.
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 1440 Human
• Background: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
• Synonyms: CSF3, GCSF, Pluripoietin, C17orf33
• Note: Centrifuge vial prior to opening.