IGF1 Rabbit Polyclonal Antibody

CAT#: PP1020B2

IGF1 rabbit polyclonal antibody, Biotin

Product Images

Sandwich ELISA using Insulin-like growth factor I / IGF1 Cat.-No PP1020B

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human IGF-I by direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I.
  Sandwich ELISA: To detect Human IGF-I by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. Used in conjunction with Polyclonal anti-Human IGF-I (Cat.-No PP1020P1 or PP1020P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I.
  Western blot: To detect Human IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Hunman IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.coli derived recombinant Human IGF-I (Cat.-No PA071)
• Specificity: This antibody reacts with Human Insulin-like Growth Factor 1.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens insulin like growth factor 1 (IGF1), transcript variant 3
• Database Link:
  Entrez Gene 3479 Human
• Background: Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.
• Synonyms: IGF-I, Somatomedin-C, Mechano growth factor, MGF, IBP1
• Note: Centrifuge vial prior to opening!

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Dilated cardiomyopathy, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Long-term depression, Melanoma, mTOR signaling pathway, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer