MCP4 (CCL13) Rabbit Polyclonal Antibody

CAT#: PP1047P2

MCP4 (CCL13) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (30.0 ng/ml), a concentration of 1.0-1.5 µg/ml of this antibody is required.
  ELISA: To detect hMCP-4 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of recombinant hMCP-4.
  Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hMCP-4.
• Specificity: Human Macrophage Chemotactic Protein-4 (MCP-4) (NCC-1).
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 6357 Human
• Background: Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes.
• Synonyms: C-C motif chemokine 13, CCL13, MCP4, NCC1, SCYA13, CK-beta-10, NCC-1, Monocyte chemotactic protein 4
• Note: Centrifuge vial prior to opening!